Neuron-Specific Chain Expression High-Molecular-Weight Clathrin
نویسندگان
چکیده
‘Departments of Pharmacy and Pharmaceutical Chemistry, School of Pharmacy, and *Department of Physiology, School of Medicine and the Howard Hughes Medical Institute, University of California, San Francisco, California 94143, 3Department of Cell Biology, Stanford University, Stanford, California 94305, and 4Department of Pathology and Laboratory Medicine, Division of Anatomic Pathology, Medical Pathology Section, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104
منابع مشابه
Clathrin light chain B: gene structure and neuron-specific splicing.
The clathrin light chains are components of clathrin coated vesicles, structural constituents involved in endocytosis and membrane recycling. The clathrin light chain B (LCB) gene encodes two isoforms, termed LCB2 and LCB3, via an alternative RNA splicing mechanism. We have determined the structure of the rat clathrin light chain B gene. The gene consists of six exons that extend over 11.9 kb. ...
متن کاملNon-stoichiometric relationship between clathrin heavy and light chains revealed by quantitative comparative proteomics of clathrin-coated vesicles from brain and liver.
We used tandem mass spectrometry with peptide counts to identify and to determine the relative levels of expression of abundant protein components of highly enriched clathrin-coated vesicles (CCVs) from rat liver. The stoichiometry of stable protein complexes including clathrin heavy chain and clathrin light chain dimers and adaptor protein (AP) heterotetramers was assessed. We detected a defic...
متن کاملTransformation by Rous sarcoma virus induces clathrin heavy chain phosphorylation
We have shown that the heavy chain of clathrin is phosphorylated in chicken embryo fibroblast cells transformed by Rous sarcoma virus, but not in normal cells. Approximately 1 mol of phosphate is bound for every 5 mol of heavy chain in the maximally phosphorylated transformed cells. Two-thirds of the phosphate is on serine and one-third on tyrosine residues. Clathrin heavy chain is a substrate ...
متن کاملAP-3: an adaptor-like protein complex with ubiquitous expression.
We have identified two closely related human proteins (sigma3A and sigma3B) that are homologous to the small chains, sigma1 and sigma2, of clathrin-associated adaptor complexes. Northern and Western blot analyses demonstrate that the products of both the sigma3A and sigma3B genes are expressed in a wide variety of tissues and cell lines. sigma3A and sigma3B are components of a large complex, na...
متن کاملMouse hepatitis virus type 2 enters cells through a clathrin-mediated endocytic pathway independent of Eps15.
It has recently been shown that cell entry of mouse hepatitis virus type 2 (MHV-2) is mediated through endocytosis (Z. Qiu et al., J. Virol. 80:5768-5776, 2006). However, the molecular mechanism underlying MHV-2 entry is not known. Here we employed multiple chemical and molecular approaches to determine the molecular pathways for MHV-2 entry. Our results showed that MHV-2 gene expression and in...
متن کامل